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Báo cáo y học: "CpG DNA modulates interleukin 1β-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "CpG DNA modulates interleukin 1β-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells. | Respiratory Research BioMed Central Research Open Access CpG DNA modulates interleukin 1 p-induced interleukin-8 expression in human bronchial epithelial 16HBE14O- cells N William Parilla1 Valerie S Hughes1 Kristin M Lierl1 Hector R Wong1 2 and Kristen Page 1 2 Address 1Division of Critical Care Medicine Cincinnati Children s Hospital Medical Center Cincinnati OH USA and 2Department of Pediatrics University of Cincinnati Cincinnati OH USA Email N William Parilla - N.william_Parilla@URMC.Rochester.edu Valerie S Hughes - valerie.strunk@cchmc.org Kristin M Lierl - kristin.lierl@cchmc.org Hector R Wong - hector.wong@cchmc.org Kristen Page - kristen.page@cchmc.org Corresponding author Published 01 June 2006 Received 27 January 2006 Respiratory Research 2006 7 84 doi l0.ll86 l465-992l-7-84 Accepted 0I June 2006 This article is available from http respiratory-research.cOm content 7 l 84 2006 Parilla et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recognition of repeat unmethylated CpG motifs from bacterial DNA through Toll-like receptor TLR-9 has been shown to induce interleukin IL -8 expression in immune cells. We sought to investigate the role of CpG oligodeoxynucleotides ODN on a human bronchial epithelial cells. Methods RT-PCR and Western blot analysis were used to determine expression of TLR-9 in human bronchial epithelial cells l6HBEl4o- . Cells were treated with CpG ODN in the presence or absence of IL-l p and IL-8 protein was determined using ELISA. In some cases cells were pretreated with chloroquine an inhibitor of TLR-9 signaling or SB202190 an inhibitor of the mitogen activated protein kinase p38 prior to treatment with IL-l p and CpG. TLR9 siRNA was used to silence TLR9 prior to treatment .