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Báo cáo y học: " Glucocorticoids activate TGF-β induced PAI-1 and CTGF expression in rat hepatocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Glucocorticoids activate TGF-β induced PAI-1 and CTGF expression in rat hepatocytes. | Comparative Hepatology BioMed Central Research Glucocorticoids activate TGF-P induced PAI-I and CTGF expression in rat hepatocytes Lucia Wickert Nicolas Chatain Karin Kruschinsky and Axel M Gressner Open Access Address Institute of Clinical Chemistry and Pathobiochemistry University Hospital RWTH Aachen Pauwelsstr. 30 D-52074 Aachen Germany Email Lucia Wickert - l_wickert@web.de Nicolas Chatain - nico@chatain.de Karin Kruschinsky-KarinKruschi@gmx.de Axel M Gressner - agressner@ukaachen.de Corresponding author Published 2 May 2007 Received 15 February 2007 Comparative Hepatology 2007 6 5 doi 10.1186 1476-5926-6-5 Accepted 2 May 2007 This article is available from http www.comparative-hepatology.cOm content 6 1 5 2007 Wickert et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In addition to the activation of hepatic stellate cells TGF-P govern apoptosis and growth control of hepatocytes in liver injury. In non-parenchymal cells TGF-P induces plasminogen activator inhibitor 1 PAI-1 and connective tissue growth factor CTGF expression which are involved in extra cellular matrix formation. Both genes were also regulated by glucocorticoids which in certain cases showed antagonistic effects to the TGF-P-Smad 3 pathway. The purpose of our work was to investigate the influence of TGF-P and dexamethasone on PAI-1 and CTGF expression and secretion in primary hepatocytes. Results By examining PAI-1 and CTGF mRNA and protein expression in cell lysates and cell-conditioned media under the influence of TGF-P and dexamethasone we analysed signalling pathways controlling their expression. TGF-P and dexamethasone significantly co-induce .