Báo cáo y học: "A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology. | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology Ryan E Campbell-Anson1 Diane Kentor1 Yi J Wang1 Kathryn M Bushnell1 Yufeng Li1 Luis M Vence1 and Laszlo G Radvanyi 1 2 Address Department of Melanoma Medical Oncology University of Texas . Anderson Cancer Center Houston TX 77030 USA and 2Department of Breast Medical Oncology University of Texas . Anderson Cancer Center Houston TX 77030 USA Email Ryan E Campbell-Anson - recampbe@ Diane Kentor - dhkentor@ Yi J Wang - yjwang@ Kathryn M Bushnell - kbushne@ Yufeng Li - yufenli@ Luis M Vence - lmvence@ Laszlo G Radvanyi - lradvanyi@ Corresponding author Published 6 March 2008 Received 15 November 2007 Journal of Immune Based Therapies and Vaccines 2008 6 1 doi 1476-8518-6-1 This article is available from http content 6 1 1 Accepted 6 March 2008 2008 Campbell-Anson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human monocyte-derived DC mDC loaded with peptides protein tumor cell lysates or tumor cell RNA are being tested as vaccines against multiple human malignancies and viral infection with great promise. One of the factors that has limited more widespread use of these vaccines is the need to generate mDC in large scale. Current methods for the large-scale cultivation of mDC in static culture vessels are labor- and time- intensive and also require many culture vessels. Here we describe a new method for the large-scale generation of human mDC from human PBMC .