Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Deletion of the meq gene significantly decreases immunosuppression in chickens caused by pathogenic marek’s disease virus | Li et al. Virology Journal 2011 8 2 http content 8 1 2 J VIROLOGY JOURNAL RESEARCH Open Access Deletion of the meq gene significantly decreases immunosuppression in chickens caused by pathogenic marek s disease virus 1 f A - 2f 2 2 -71 I Z--2 1 Yanpeng Li Aijun Sun Shuai Su Peng Zhao Zhizhong Cui Hongfei Zhu Abstract Background Marek s disease virus MDV causes an acute lymphoproliferative disease in chickens resulting in immunosuppression which is considered to be an integral aspect of the pathogenesis of Marek s disease MD . A recent study showed that deletion of the Meq gene resulted in loss of transformation of T-cells in chickens and a Meq-null virus rMd5AMeq could provide protection superior to CVI988 Rispens. Results In the present study to investigate whether the Meq-null virus could be a safe vaccine candidate we constructed a Meq deletion strain GX0101AMeq by deleting both copies of the Meq gene from a pathogenic MDV GX0101 strain which was isolated in China. Pathogenesis experiments showed that the GX0101AMeq virus was fully attenuated in specific pathogen-free chickens because none of the infected chickens developed Marek s disease-associated lymphomas. The study also evaluated the effects of GX0101AMeq on the immune system in chickens after infection with GX0101AMeq virus. Immune system variables including relative lymphoid organ weight blood lymphocytes and antibody production following vaccination against AIV and NDV were used to assess the immune status of chickens. Experimental infection with GX0101AMeq showed that deletion of the Meq gene significantly decreased immunosuppression in chickens caused by pathogenic MDV. Conclusion These findings suggested that the Meq gene played an important role not only in tumor formation but also in inducing immunosuppressive effects in MDV-infected chickens. Background Marek s disease MD is a neoplastic disease of chickens which is caused by the lymphotropic alphaherpesvirus MD virus MDV . MD is
