Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea | Meijón et al. BMC Plant Biology 2010 10 10 http 1471-2229 10 10 BMC Plant Biology RESEARCH ARTICLE Open Access Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea Mónica Meijón1 2 Isabel Feito3 Luis Valledor1 2 Roberto Rodríguez1 2 María Jesus Canal1 2 Abstract Background The ability to control the timing of flowering is a key strategy for planning production in ornamental species such as azalea however it requires a thorough understanding of floral transition. Floral transition is achieved through a complex genetic network and regulated by multiple environmental and endogenous cues. Dynamic changes between chromatin states facilitating or inhibiting DNA transcription regulate the expression of floral induction pathways in response to environmental and developmental signals. DNA methylation and histone modifications are involved in controlling the functional state of chromatin and gene expression. Results The results of this work indicate that epigenetic mechanisms such as DNA methylation and histone H4 acetylation have opposite and particular dynamics during the transition from vegetative to reproductive development in the apical shoots of azalea. Global levels of DNA methylation and histone H4 acetylation as well as immunodetection of 5-mdC and acetylated H4 in addition to a morphological study have permitted the delimitation of four basic phases in the development of the azalea bud and allowed the identification of a stage of epigenetic reprogramming which showed a sharp decrease of whole DNA methylation similar to that is defined in other developmental processes in plants and in mammals. Conclusion The epigenetic control and reorganization of chromatin seem to be decisive for coordinating floral development in azalea. DNA methylation and H4 deacetylation act simultaneously and co-ordinately restructuring the chromatin and regulating the gene expression during soot apical meristem development .
