Refers to a natural process converting atmospheric nitrogen to ammonium to yield usable nitrogen source. • Only a few prokaryotic species can "fix" nitrogen. • Nitrogenase complex dinitrogenase dinitrogenase reductase | Subject: Biochemistry Instructor: MSc. Le Hong Phu Day: 2 Period: 1234 Semester 2 Academic year: 2009-2010 Group 4 OVERVIEW ON NITROGEN METABOLISM: SYNTHESIS GROUP MEMBERS Nguyễn Thị Thúy Giang BTIU08047 Chung Thế Hào BTUN08015 Lại Nguyên Huy BTIU08015 Nguyễn Quang Huy BTWE08012 Đặng Xuân Khải BTIU08019 Dương Bá Phú BTIU08058 Trần Triệu Quân BTUN08027 Đường Thị Kiều Trang BTWE08 Huỳnh Hải Vân BTIU08 OUTLINE Introduction Body Nitrogen fixation Transamination reaction and incorporation of ammonium Biosynthesis of amino acids Glutamate Aspartate Serine Pyruvate Aromatic Histidine One carbon metabolism Amino acids as metabolic precursor Glutathione Alkaloid Nucleotides Heme group 6. Regulation of amino acid biosynthesis Conclusion INTRODUCTION WHY CARES? Nitrogenous compounds are prevalent. Nitrogenous compounds are metabolic important Determine our health. Human alone cannot synthesize all these compounds. Only some forms of nitrogen are accessible WHAT WE FOCUS? The “build up” of . | Subject: Biochemistry Instructor: MSc. Le Hong Phu Day: 2 Period: 1234 Semester 2 Academic year: 2009-2010 Group 4 OVERVIEW ON NITROGEN METABOLISM: SYNTHESIS GROUP MEMBERS Nguyễn Thị Thúy Giang BTIU08047 Chung Thế Hào BTUN08015 Lại Nguyên Huy BTIU08015 Nguyễn Quang Huy BTWE08012 Đặng Xuân Khải BTIU08019 Dương Bá Phú BTIU08058 Trần Triệu Quân BTUN08027 Đường Thị Kiều Trang BTWE08 Huỳnh Hải Vân BTIU08 OUTLINE Introduction Body Nitrogen fixation Transamination reaction and incorporation of ammonium Biosynthesis of amino acids Glutamate Aspartate Serine Pyruvate Aromatic Histidine One carbon metabolism Amino acids as metabolic precursor Glutathione Alkaloid Nucleotides Heme group 6. Regulation of amino acid biosynthesis Conclusion INTRODUCTION WHY CARES? Nitrogenous compounds are prevalent. Nitrogenous compounds are metabolic important Determine our health. Human alone cannot synthesize all these compounds. Only some forms of nitrogen are accessible WHAT WE FOCUS? The “build up” of nitrogenous compounds (monomer only): amino acids and more. The biosynthesis pathways are extensively diverse. Nitrogen balance: Positive: intake > loss childhood, pregnancy, illness recovery. Negative : intake All species that can fix nitrogen possess the nitrogenase complex. Its structure, similar in all species so far investigated, consists of two proteins called dinitrogenase and dinitrogenase reductase. Dinitrogenase: Referred to as Fe-Mo protein. Heterotetramerous, containing two Mo atoms, and 30 iron atoms. Catalyze the reaction: N2 + 8H+ + 8e- 2NH3 + H2 Dinitrogenase reductase: Referred to as Fe protein. is a dimer Carry electrons from ferrodoxin and integrate with ATPs. 16 ATPs are spent
