In India, around 93 tobacco varieties were released and three cultivars identified for commercial cultivation in 10 types of tobacco grown under various agro climatic regions. In order to make this information available to researchers, farmers and students, an online expert system on tobacco varieties was developed using Agridaksh tool designed for creation of knowledge base of all crops at a single platform for global accessing. The information on tobacco varieties provided by the domain experts of tobacco crop was used in constructing the knowledge model.