Handbook of Microbiological Media, Fourth Edition part 6 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Achromobacter Medium 45 PPLO broth without Crystal Fresh yeast extract pH at 25 C PPLO Broth without Crystal Violet Composition per Beef heart infusion Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Fresh Yeast Extract Solution Composition per Baker s yeast live pressed Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to of distilled deionized water. Mix thoroughly. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to . Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into test tubes or flasks. Autoclave for 10 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Acholeplasma species. Acholeplasma Medium ATCC Medium 1215 Composition per PPLO broth without Crystal Fetal bovine serum heat Fresh yeast extract Tween -glucose-BSA Phenol Red solution . PPLO Broth without Crystal Violet Composition per Beef heart infusion Peptone. Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to . Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Fresh Yeast Extract Solution Composition per Baker s yeast live pressed Preparation of Fresh Yeast Extract Solution Add the .
