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Candida infections detection and epidemiology - part 8

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Trong tất cả các phân lập thu được từ bệnh viêm phổi đã được thử nghiệm trong khảo nghiệm proteinase (n = 23), 96% sản xuất một lượng đáng kể của các proteinase (+ hoặc + +), trong khi đối với các phân lập thu được từ các nguồn khác (máu (n = 131), đường tiết niệu (n = 24) | High levels of hydrolytic enzymes Proteinase assay. One-hundred-and-eighty-five isolates were tested in de proteinase assay. The number of isolates and the different scores are depicted in Table 5. In all but nine isolates proteinase activity was detected 95 . Several isolates were tested in duplicate or triplicate and showed only minor differences average difference between duplicate tests 0.03 . There was no clear correlation between the results of the proteinase assay and the geographical distribution of the isolates. Of all isolates obtained from pneumonia that were tested in the proteinase assay n 23 96 produced considerable amounts of proteinase or whereas for isolates obtained from the other sources blood n 131 the urinary tract n 24 or wound sst n 7 this percentage was 80 79 and 73 respectively Table 6 . However this difference was not statistically significant. Table 5 Results of the proteinase assay Score - - No. isolates 9 5 25 14 118 64 33 18 Table 6 Results of the proteinase assay in relation with the site of infection No. of isolates Source - - Total blood 5 4 21 16 83 63 22 17 131 100 pneumonia 0 0 1 4 14 61 8 35 23 100 urinary tract 2 8 3 13 17 71 2 8 24 100 wound s st 2 29 0 0 4 57 1 14 7 100 wound s st isolates originating from wounds skin or soft tissue Discussion Previous studies on the phospho lipase activity of C. albicans isolates reported a large variation in activity among different isolates but a remarkably constant degree of activity of individual isolates which was fairly independent of inoculum size10 14 16. This is in agreement with our results the ratio of the diameter of the colony plus precipitation zone and the colony alone ranged from 1.05 to 2.36 in the positive isolates with an average difference between duplicate tests of 0.08. When looking at phospho lipase activity in relation to the site of infection Price et al. found that 55 of the blood isolates studied were positive in the assay14. These isolates were also among the .