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Báo cáo y học: "Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation. | Wu et al. Journal of Hematology Oncology 2011 4 19 http www.jhoonline.Org content 4 1 19 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation 1.2 1 P s 3 1 I 1 4 2 1 Xiuli Wu Kanger Zhu Xin Du Shaohua Chen Lijian Yang Jufeng Wu Qifa Liu and Yangqiu Li Abstract Background Allogeneic hematopoietic stem cell transplantation allo-HSCT leads to a prolonged state of immunodeficiency and requires reconstitution of normal T-cell immunity. Signal joint T-cell receptor excision DNA circles sjTRECs are markers of developmental proximity to the thymus that have been used to evaluate thymic function related to T-cell immune reconstitution after HSCT. To assess the proliferative history in different T-cell receptor beta variable region TRBV subfamilies of T cells after HSCT expansion of TRBV subfamily-naive T cells was determined by analysis of a series of TRBV-BD1 sjTRECs. Methods sjTRECs levels were detected by real-time quantitative polymerase chain reaction PCR in peripheral blood mononuclear cells PBMCs from 43 Chinese acute leukemia patients who underwent allo-HSCT. Twenty-three TRBV-BD1 sjTRECs were amplified by semi-nested PCR. Sixteen age-matched healthy volunteers served as normal controls. Results sjTRECs levels were low or undetectable in the first 6 weeks after allo-HSCT and increased after 8 weeks post HSCT however sjTRECs levels at week 20 post-HSCT were still less than normal controls. Frequencies of TRBV subfamily sjTRECs in PBMCs from recipients at week 8 post-HSCT 29.17 20.97 or at week 16 post-HSCT 38.33 9.03 were significantly lower than those in donors 47.92 13.82 or recipients at pre-HSCT 45.83 14.03 . However frequencies of TRBV subfamily sjTRECs in recipients at week 30 post-HSCT 42.71 21.62 were similar to those in donors and recipients at pre-HSCT. sjTRECs levels in donors had a positive .