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báo cáo khoa học: "Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia | Wang et al. Journal of Hematology Oncology 2010 3 14 http www.jhoonline.Org content 3 1 14 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia 1 1 2 2 2 2 2.3 Liang Wang Kanger Zhu Xianfeng Zha Shaohua Chen Lijian Yang Si Chen Yangqiu Li Abstract Introduction The development of Philadelphia chromosome Ph negative acute leukemia myelodysplastic syndrome MDS in patients with Ph-positive chronic myeloid leukemia CML is very rare. The features of restrictive usage and absence of partial T cell clones have been found in patients with CML. However the T-cell clonal evolution of Ph-negative malignancies during treatment for CML is still unknown. Objective To investigate the dynamic change of clonal proliferation ofT cell receptor TCR Va and Vb subfamilies in one CML patient who developed Ph-negative acute lymphoblastic leukemia ALL after interferon and imatinib therapy. Methods The peripheral blood mononuclear cells PBMC samples were collected at the 3 time points diagnosis of Ph-positive chronic phase CP CML developing Ph-negative ALL and post inductive chemotherapy CT for Ph-negative ALL respectively . The CDR3 size of TCR Va and Vb repertoire were detected by RT-PCR. The PCR products were further analyzed by genescan to identify T cell clonality. Results The CML patient who achieved complete cytogenetic remission CCR after 5 years of IFN-a therapy suddenly developed Ph-negative ALL 6 months following switch to imatinib therapy. The expression pattern and clonality of TCR Va Vb T cells changed in different disease stages. The restrictive expression of Va Vb subfamilies could be found in all three stages and partial subfamily of T cells showed clonal proliferation. Additionally there have been obvious differences in Va Vb subfamily of T cells between the stages of Ph-positive CML-CP and Ph-negative