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Báo cáo y học: "Early Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages. | Journal of Inflammation BioMed Central Research Open Access Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages Sandra Olsson and Roger Sundler Address Department of Experimental Medical Science Lund University BMC B12 SE-22184 Lund Sweden Email Sandra Olsson - Sandra.Olsson@med.lu.se Roger Sundler - Roger.Sundler@med.lu.se Corresponding author Published 04 May 2006 Received 05 October 2005 Journal of Inflammation 2006 3 8 doi 10.1 186 1476-9255-3-8 Accepted 04 May 2006 This article is available from http www.journal-inflammation.cOm content 3 1 8 2006 Olsson and Sundler licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Yeast and bacteria elicit arachidonate release in macrophages leading to the formation of leukotrienes and prostaglandins important mediators of inflammation. Receptors recognising various microbes have been identified but the signalling pathways are not entirely understood. Cytosolic phospholipase A2 is a major down-stream target and this enzyme is regulated by both phosphorylation and an increase in intracellular Ca2 . Potential signal components are MAP kinases phosphatidylinositol 3-kinase and phospholipase Cỵ2. The latter can undergo tyrosine phosphorylation and Src family kinases might carry out this phosphorylation. Btk a Tec family kinase could also be important. Our aim was to further elucidate the role of Src family kinases and Btk. Methods Arachidonate release from murine peritoneal macrophages was measured by prior radiolabeling. Furthermore immunoprecipitation and Western blotting were used to monitor changes in activity phosphorylation of intermediate signal components. To determine the role of .