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báo cáo khoa học: " Assessing the contribution of alternative splicing to proteome diversity in Arabidopsis thaliana using proteomics data"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Assessing the contribution of alternative splicing to proteome diversity in Arabidopsis thaliana using proteomics data | Severing et al. BMC Plant Biology 2011 11 82 http www.biomedcentral.com 1471-2229 11 82 BMC Plant Biology CORRECTION Open Access Assessing the contribution of alternative splicing to proteome diversity in Arabidopsis thaliana using proteomics data Edouard I Severing1 2 3 Aalt DJ van Dijk1 and Roeland CHJ van Ham1 2 3 4 Abstract Background Large-scale analyses of genomics and transcriptomics data have revealed that alternative splicing AS substantially increases the complexity of the transcriptome in higher eukaryotes. However the extent to which this complexity is reflected at the level of the proteome remains unclear. On the basis of a lack of conservation of AS between species we previously concluded that AS does not frequently serve as a mechanism that enables the production of multiple functional proteins from a single gene. Following this conclusion we hypothesized that the extent to which AS events contribute to the proteome diversity in Arabidopsis thaliana would be lower than expected on the basis of transcriptomics data. Here we test this hypothesis by analyzing two large-scale proteomics datasets from Arabidopsis thaliana. Results A total of only 60 AS events could be confirmed using the proteomics data. However for about 60 of the loci that based on transcriptomics data were predicted to produce multiple protein isoforms through AS no isoform-specific peptides were found. We therefore performed in silico AS detection experiments to assess how well AS events were represented in the experimental datasets. The results of these in silico experiments indicated that the low number of confirmed AS events was the consequence of a limited sampling depth rather than in vivo under-representation of AS events in these datasets. Conclusion Although the impact of AS on the functional properties of the proteome remains to be uncovered the results of this study indicate that AS-induced diversity at the transcriptome level is also expressed at the proteome level. .