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báo cáo khoa học: " Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (Arachis hypogaea L)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (Arachis hypogaea L) | Wang et al. BMC Plant Biology 2010 10 267 http www.biomedcentral.com 1471-2229 10 267 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut Arachis hypogaea L Tong Wang1 2 Erhua Zhang2 Xiaoping Chen2 Ling Li1 Xuanqiang Liang1 2 Abstract Background Pre-harvest infection of peanuts by Aspergillus flavus and subsequent aflatoxin contamination is one of the food safety factors that most severely impair peanut productivity and human and animal health especially in arid and semi-arid tropical areas. Some peanut cultivars with natural pre-harvest resistance to aflatoxin contamination have been identified through field screening. However little is known about the resistance mechanism which has slowed the incorporation of resistance into cultivars with commercially acceptable genetic background. Therefore it is necessary to identify resistance-associated proteins and then to recognize candidate resistance genes potentially underlying the resistance mechanism. Results The objective of this study was to identify resistance-associated proteins in response to A. flavus infection under drought stress using two-dimensional electrophoresis with mass spectrometry. To identify proteins involved in the resistance to pre-harvest aflatoxin contamination we compared the differential expression profiles of seed proteins between a resistant cultivar YJ-1 and a susceptible cultivar Yueyou 7 under well-watered condition drought stress and A. flavus infection with drought stress. A total of 29 spots showed differential expression between resistant and susceptible cultivars in response to A. flavus attack under drought stress. Among these spots 12 protein spots that consistently exhibited an altered expression were screened by Image Master 5.0 software and successfully identified by MALDI-TOF MS. Five protein spots including Oso7g0179400 PII protein CDK1 Oxalate oxidase SAP domain-containing