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báo cáo khoa học: " Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production | Gardiner et al. BMC Plant Biology 2010 10 289 http www.biomedcentral.com 1471-2229 10 289 BMC Plant Biology RESEARCH ARTICLE Open Access Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production 1 1 2 2 1 1 Donald M Gardiner Kemal Kazan Sebastien Praud Francois J Torney Anca Rusu John M Manners Abstract Background The fungal pathogen Fusarium graminearum causes Fusarium Head Blight FHB disease on wheat which can lead to trichothecene mycotoxin e.g. deoxynivalenol DON contamination of grain harmful to mammalian health. DON is produced at low levels under standard culture conditions when compared to plant infection but specific polyamines e.g. putrescine and agmatine and amino acids e.g. arginine and ornithine are potent inducers of DON by F. graminearum in axenic culture. Currently host factors that promote mycotoxin synthesis during FHB are unknown but plant derived polyamines could contribute to DON induction in infected heads. However the temporal and spatial accumulation of polyamines and amino acids in relation to that of DON has not been studied. Results Following inoculation of susceptible wheat heads by F. graminearum DON accumulation was detected at two days after inoculation. The accumulation of putrescine was detected as early as one day following inoculation while arginine and cadaverine were also produced at three and four days post-inoculation. Transcripts of ornithine decarboxylase ODC and arginine decarboxylase ADC two key biosynthetic enzymes for putrescine biosynthesis were also strongly induced in heads at two days after inoculation. These results indicated that elicitation of the polyamine biosynthetic pathway is an early response to FHB. Transcripts for genes encoding enzymes acting upstream in the polyamine biosynthetic pathway as well as those of ODC and ADC and putrescine levels were also induced in the rachis a flower organ supporting DON production