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báo cáo khoa học: " Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA) | Xu et al. BMC Plant Biology 2011 11 38 http www.biomedcentral.com 1471-2229 11 38 BMC Plant Biology RESEARCH ARTICLE Open Access Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana Musa spp. AAA Z-I 1t vt-i v2 3 3 2 Chunxiang Xu Tomás Takác Christian Burbach Diedrik Menzel Jozef Samaj Abstract Background Hydroxyproline rich glycoproteins HRGPs are implicated to have a role in many aspects of plant growth and development but there is limited knowledge about their localization and function during somatic embryogenesis of higher plants. In this study the localization and function of hydroxyproline rich glycoproteins in embryogenic cells ECs and somatic embryos of banana were investigated by using immunobloting and immunocytochemistry with monoclonal JIM11 and JIM20 antibodies as well as by treatment with 3 4-dehydro-L-proline 3 4-DHP an inhibitor of extensin biosynthesis and by immunomodulation with the JIM11 antibody. Results Immunofluorescence labelling of JIM11 and JIM20 hydroxyproline rich glycoprotein epitopes was relatively weak in non-embryogenic cells NECs mainly on the edge of small cell aggregates. On the other hand hydroxyproline rich glycoprotein epitopes were found to be enriched in early embryogenic cells as well as in various developmental stages of somatic embryos. Embryogenic cells ECs proembryos and globular embryos showed strong labelling of hydroxyproline rich glycoprotein epitopes especially in their cell walls and outer surface layer so-called extracellular matrix ECM . This hydroxyproline rich glycoprotein signal at embryo surfaces decreased and or fully disappeared during later developmental stages e.g. pear-shaped and cotyledonary stages of embryos. In these later developmental embryogenic stages however new prominent hydroxyproline rich glycoprotein labelling appeared in tri-cellular junctions among parenchymatic cells inside these embryos. Overall immunofluorescence labelling of