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báo cáo khoa học: " Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.) | Bohra et al. BMC Plant Biology 2011 11 56 http www.biomedcentral.com 1471-2229 11 56 BMC Plant Biology RESEARCH ARTICLE Open Access Analysis of BAC-end sequences BESs and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea Cajanus spp. Abhishek Bohra1 2t Anuja Dubey1 3t Rachit K Saxena1 2 R Varma Penmetsa4 KN Poornima1 5 Naresh Kumar1 6 Andrew D Farmer7 Gudipati Srivani1 Hari D Upadhyaya1 Ragini Gothalwal3 S Ramesh5 Dhiraj Singh6 m ilhibi 1 PR W i l icbcr2 i l IZ Rir-icih8 m _ - . . 9 m ỉt lsrv7 Kulbhushan Saxena PB Kavi Kisiioi Nagendia K Singh Cinistopiiei D Town Giegoiy D May Douglas R Cook4 and Rajeev K Varshney1 10 Abstract Background Pigeonpea Cajanus cajan L. Millsp. is an important legume crop of rainfed agriculture. Despite of concerted research efforts directed to pigeonpea improvement stagnated productivity of pigeonpea during last several decades may be accounted to prevalence of various biotic and abiotic constraints and the situation is exacerbated by availability of inadequate genomic resources to undertake any molecular breeding programme for accelerated crop improvement. With the objective of enhancing genomic resources for pigeonpea this study reports for the first time large scale development of SSR markers from BAC-end sequences and their subsequent use for genetic mapping and hybridity testing in pigeonpea. Results A set of 88 860 BAC bacterial artificial chromosome -end sequences BESs were generated after constructing two BAC libraries by using Hindlll 34 560 clones and BamHI 34 560 clones restriction enzymes. Clustering based on sequence identity of BESs yielded a set of 52K non-redundant sequences comprising 35 Mbp or 4 of the pigeonpea genome. These sequences were analyzed to develop annotation lists and subdivide the BESs into genome fractions e.g. genes retroelements transpons and non-annotated sequences . Parallel analysis of BESs for microsatellites or simple sequence repeats SSRs identified 18 .