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báo cáo khoa học: " A compatible interaction of Alternaria brassicicola with Arabidopsis thaliana ecotype DiG: evidence for a specific transcriptional signature"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A compatible interaction of Alternaria brassicicola with Arabidopsis thaliana ecotype DiG: evidence for a specific transcriptional signature | BMC Plant Biology BioMed Central Open Access Research article A compatible interaction of Alternaria brassicicola with Arabidopsis thaliana ecotype DiG evidence for a specific transcriptional signature Arup K Mukherjee1 Sophie Lev2 Shimon Gepstein2 and Benjamin A Horwitz 2 Address division of Plant Biotechnology Regional Plant Resource Centre IRC Village Bhubaneswar 751015 Orissa India and 2Department of Biology Israel Institute of Technology Technion Haifa 32000 Israel Email Arup K Mukherjee - titirtua@gmail.com Sophie Lev-levsophie@gmail.com Shimon Gepstein - gepstein@tx.technion.ac.il Benjamin A Horwitz - horwitz@tx.technion.ac.il Corresponding author Published 18 March 2009 BMC Plant Biology 2009 9 31 doi l0.ll86 l47l-2229-9-3l Received 12 December 2008 Accepted 18 March 2009 This article is available from http www.biomedcentral.cOm 1471-2229 9 3 1 2009 Mukherjee et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The interaction of Arabidopsis with Alternaria brassicicola provides a model for disease caused by necrotrophs but a drawback has been the lack of a compatible pathosystem. Infection of most ecotypes including the widely-studied line Col-0 with this pathogen generally leads to a lesion that does not expand beyond the inoculated area. This study examines an ecotype Dijon G DiG which is considered sensitive to A. brassicicola. Results We show that the interaction has the characteristics of a compatible one with expanding rather than limited lesions. To ask whether DiG is merely more sensitive to the pathogen or rather interacts in distinct manner we identified genes whose regulation differs between Col-0 and DiG challenged with A. brassicicola. Suppression subtractive hybridization was used