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báo cáo khoa học: " Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa) | BMC Plant Biology BioMed Central Research article Open Access Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice Oryza sativa Qian-Hao Zhu Narayana M Upadhyaya Frank Gubler and Chris A Helliwell Address CSIRO Plant Industry GPO Box 1600 Canberra ACT 2601 Australia Email Qian-Hao Zhu - qianhao.zhu@csiro.au Narayana M Upadhyaya - narayana.upadhyaya@csiro.au Frank Gubler - frank.gubler@csiro.au Chris A Helliwell - chris.helliwell@csiro.au Corresponding author Published 17 December 2009 Received 29 July 2009 BMC Plant Biology 2009 9 149 doi 10.1186 1471-2229-9-149 Accepted I7 December 2009 This article is available from http www.biomedcentral.cOm 1471-2229 9 149 2009 Zhu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Regulation of gene expression by microRNAs miRNAs plays a crucial role in many developmental and physiological processes in plants. miRNAs act to repress expression of their target genes via mRNA cleavage or translational repression. Dozens of miRNA families have been identified in rice 21 of which are conserved between rice and Arabidopsis. miR172 is a conserved miRNA family which has been shown to regulate expression of APETALA2 AP2 -like transcription factors in Arabidopsis and maize. The rice genome encodes five AP2-like genes predicted to be targets of miR172. To determine whether these rice AP2-like genes are regulated by miR172 and investigate the function of the target genes we studied the effect of over-expressing two members of the miR172 family on rice plant development. Results Analysis of miR172 expression showed that it is most highly expressed in late vegetative stages and developing panicles. Analyses of expression of three