Đang chuẩn bị liên kết để tải về tài liệu:
báo cáo khoa học: " Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways | Weisman et al. BMC Plant Biology 2010 10 59 http www.biomedcentral.com 1471-2229 10 59 BMC Plant Biology RESEARCH ARTICLE _ Open Access Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways David Weisman11 Merianne Alkiof2 and Adán Colón-Carmona 1 Abstract Background Polycyclic aromatic hydrocarbons PAHs are toxic widely-distributed environmentally persistent and carcinogenic byproducts of carbon-based fuel combustion. Previously plant studies have shown that PAHs induce oxidative stress reduce growth and cause leaf deformation as well as tissue necrosis. To understand the transcriptional changes that occur during these processes we performed microarray experiments on Arabidopsis thaliana L. under phenanthrene treatment and compared the results to published Arabidopsis microarray data representing a variety of stress and hormone treatments. In addition to probe hormonal aspects of PAH stress we assayed transgenic ethyleneinducible reporter plants as well as ethylene pathway mutants under phenanthrene treatment. Results Microarray results revealed numerous perturbations in signaling and metabolic pathways that regulate reactive oxygen species ROS and responses related to pathogen defense. A number of glutathione S-transferases that may tag xenobiotics for transport to the vacuole were upregulated. Comparative microarray analyses indicated that the phenanthrene response was closely related to other ROS conditions including pathogen defense conditions. The ethylene-inducible transgenic reporters were activated by phenanthrene. Mutant experiments showed that PAH inhibits growth through an ethylene-independent pathway as PAH-treated ethylene-insensitive etr1-4 mutants exhibited a greater growth reduction than WT. Further phenanthrene-treated constitutive ethylene signaling mutants had longer roots than the untreated control plants indicating that the PAH .