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báo cáo khoa học: " Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae | Wu et al. BMC Plant Biology 2010 10 68 http www.biomedcentral.com 1471-2229 10 68 BMC Plant Biology RESEARCH ARTICLE _ Open Access Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae Fu-Hui Wu 1 Ming-Tsair Chan21 De-Chih Liao 1 Chen-Tran Hsu1 Yi-Wei Lee1 Henry Daniell2 Melvin R Duvall3 and Choun-Sea Lin 1 Abstract Background Oncidium spp. produce commercially important orchid cut flowers. However they are amenable to intergeneric and inter-specific crossing making phylogenetic identification very difficult. Molecular markers derived from the chloroplast genome can provide useful tools for phylogenetic resolution. Results The complete chloroplast genome of the economically important Oncidium variety Onc. Gower Ramsey Accession no. GQ324949 was determined using a polymerase chain reaction PCR and Sanger based ABI sequencing. The length of the Oncidium chloroplast genome is 146 484 bp. Genome structure gene order and orientation are similar to Phalaenopsis but differ from typical Poaceae other monocots for which there are several published chloroplast cp genome. The Onc. Gower Ramsey chloroplast-encoded NADH dehydrogenase ndh genes except ndhE lack apparent functions. Deletion and other types of mutations were also found in the ndh genes of 15 other economically important Oncidiinae varieties except ndhE in some species. The positions of some species in the evolution and taxonomy of Oncidiinae are difficult to identify. To identify the relationships between the 15 Oncidiinae hybrids eight regions of the Onc. Gower Ramsey chloroplast genome were amplified by PCR for phylogenetic analysis. A total of 7042 bp derived from the eight regions could identify the relationships at the species level which were supported by high bootstrap values. One particular 1846 bp region derived from two PCR products trnHGUG -psbA and trnFGAA-ndhJ was adequate for correct phylogenetic placement of 13 of the .