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Báo cáo y học: "Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages | Revie et al. Virology Journal 2010 7 155 http www.virologyj.eom content 7 1 155 VIROLOGY JOURNAL RESEARCH Open Access Analysis of the 5 UTR of HCV genotype 3 grown in vitro in human B cells T cells and macrophages Dennis Revie1 Michael O Alberti1 5 John G Prichard2 Ann S Kelley3 and S Zaki Salahuddin 4 Abstract Background Previously we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 HCV-1 from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 HCV-3 as diagnosed by clinical laboratories. Results HCV was cultured in vitro using our system. HCV RNA was isolated from patients blood and from HCV cultured in various cell types for up to three months. The 5 UTR of these isolates were used for comparisons. Results revealed a number of sequence changes as compared to the serum RNA. The HCV RNA produced efficiently by infected macrophages B-cells and T-cells had sequences similar to HCV-1 which suggests that selection of the variants was performed at the level of macrophages. Virus with sequences similar to HCV-1 replicated better in macrophages than HCV having a 5 UTR similar to HCV-3. Conclusions Although HCV-3 replicates in cell types such as B-cells T-cells and macrophages it may require a different primary cell type for the same purpose. Therefore in our opinion HCV-3 does not replicate efficiently in macrophages and patients infected with HCV-3 may contain a population of HCV-1 in their blood. Background HCV is associated with a number of diseases including hepatocellular carcinoma B-cell lymphomas and neuropathy. There is an emerging list of diseases that may have some association with this virus. Approximately 8 of HCV-infected individuals in the United States are infected with genotype 3 1 . The chances of liver damage due to HCV infections may not vary by genotype in untreated individuals 2 3 and infections with HCV-3 are more likely to respond earlier to .