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Báo cáo khoa học: "Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach | Virology Journal BioMed Central Research Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach Megan Moerdyk-Schauwecker1 Sun-Il Hwang2 and Valery Z Grdzelishvili 1 Address Department of Biology University of North Carolina at Charlotte Charlotte NC 28223 USA and 2Cannon Research Center Carolinas Medical Center Charlotte NC 28203 USA Email Megan Moerdyk-Schauwecker - mmoerdyk@uncc.edu Sun-Il Hwang - Sunil.Hwang@carolinashealthcare.org Valery Z Grdzelishvili - vzgrdzel@uncc.edu Corresponding author Open Access Published 12 October 2009 Received 20 August 2009 Accepted 12 October 2009 Virology Journal 2009 6 166 doi l0.ll86 I743-422X-6-I66 This article is available from http www.virologyj.com content 6 1 166 2009 Moerdyk-Schauwecker et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Vesicular stomatitis virus VSV is the prototypic rhabdovirus and the best studied member of the order Mononegavirales. There is now compelling evidence that enveloped virions released from infected cells carry numerous host cellular proteins some of which may play an important role in viral replication. Although several cellular proteins have been previously shown to be incorporated into VSV virions no systematic study has been done to reveal the host protein composition for virions of VSV or any other member of Mononegavirales. Results Here we used a proteomics approach to identify cellular proteins within purified VSV virions thereby creating a snapshot of one stage of virus host interaction that can guide future experiments aimed at understanding molecular mechanisms of virus-cell interactions. Highly purified preparations of VSV virions from three different cell lines of .