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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR | Virology Journal BioMed Central Open Access Methodology A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR Nina Jonsson Maria Gullberg Stina Israelsson and A Michael Lindberg Address School of Pure and Applied Natural Sciences University of Kalmar SE-391 82 Kalmar Sweden Email Nina Jonsson - nina.jonsson@hik.se Maria Gullberg - maria.gullberg@hik.se Stina Israelsson - stina.israelsson@hik.se A Michael Lindberg - michael.lindberg@hik.se Corresponding author Published 7 December 2009 Virology Journal 2009 6 217 doi l0.ll86 l743-422X-6-2l 7 Received 4 September 2009 Accepted 7 December 2009 This article is available from http www.virologyj.Com content 6 l 2l7 2009 Jonsson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Measuring virus attachment to host cells is of great importance when trying to identify novel receptors. The presence of a usable receptor is a major determinant of viral host range and cell tropism. Furthermore identification of appropriate receptors is central for the understanding of viral pathogenesis and gives possibilities to develop antiviral drugs. Attachment is presently measured using radiolabeled and subsequently gradient purified viruses. Traditional methods are expensive and time-consuming and not all viruses are stable during a purification procedure hence there is room for improvement. Real-time PCR RT-PCR has become the standard method to detect and quantify virus infections including enteroviruses in clinical samples. For instance primers directed to the highly conserved 5 untranslated region 5 UTR of the enterovirus genome enable detection of a wide spectrum of enteroviruses. Here we evaluate the