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Báo cáo y học: "Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4+ T cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4+ T cells | Patil et al. Virology Journal 2010 7 3 http www.virologyj.eom content 7 1 3 VIROLOGY JOURNAL SHORT REPORT Open Access Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4 T cells Ajit Patil Archana Gautam Jayanta Bhattacharya Abstract HIV-1 particle assembly mediated by viral Gag protein occurs predominantly at plasma membrane. While colocalization of HIV-1 envelope with lipid rich microenvironment have been shown in T cells the significance of viral proteins modulating envelope association in such microdomains in plasma membrane enriched in glycosylphosphatidylinositol-anchored proteins in primary CD4 T cells that are natural targets of HIV-1 is poorly understood. Here we show that in primary CD4 T cells that are natural targets of HIV-1 in vivo Gag modulates HIV-1 envelope association with GM1 ganglioside and CD59 rich cellular compartments as well as with detergent resistant membranes. Our data strengthen evidence that Gag-Env interaction is important in envelope association with lipid rafts containing GPI-anchored proteins for efficient assembly onto mature virions resulting in productive infection of primary CD4 T cells. Findings Human Immunodeficiency Virus Type 1 HIV-1 has been shown to assemble via specialized plasma membrane domains popularly known as lipid rafts 1-6 which are rich in cholesterol and sphingomyelin within an ordered structure and plays important role in cell signaling 7 . Rafts are believed to play an important role towards facilitating HIV- 1 assembly particularly exploiting acylated residues in viral Gag 1 3 5 and envelope Env 8 9 . However the precise mechanism by which lipid rafts functions in targeting viral Env and Gag to the plasma membrane in infected T cells and facilitate assembly is not clearly understood. Previously Jolly and Sattentau 10 have shown that raft integrity is critical for Env