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báo cáo khoa học: " Rapid EST isolation from chromosome 1R of rye"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid EST isolation from chromosome 1R of rye | BioMed Central BMC Plant Biology Research article Open Access Rapid EST isolation from chromosome IR of rye Ruo-Nan Zhou1 4 Rui Shi1 5 Shu-Mei Jiang1 2 Wei-Bo Yin1 Huang-Huang Wang1 Yu-Hong Chen1 Jun Hu1 Richard RC Wang3 Xiang-Qi Zhang1 and Zan-Min Hu 1 Address 1Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 P. R. China 2South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 P. R. China 3USDA-ARS FRRL Utah State University Logan UT 84322-6300 USA 4Graduate University of Chinese Academy of Sciences Beijing 100049 P. R. China and 5Forest Biotechnology Group N.C. State University Campus Box 7247 Raleigh NC 27695-7247 UsA Email Ruo-Nan Zhou - wawazhoujian@163.com Rui Shi - rshi@ncsu.edu Shu-Mei Jiang - shmjiang@163.com Wei- Bo Yin - wbyin@genetics.ac.cn Huang-Huang Wang - huanhuanbj@hotmail.com Yu-Hong Chen - yhchen@genetics.ac.cn Jun Hu - jhu@genetics.ac.cn Richard RC Wang - richard.wang@ars.usda.gov Xiang-Qi Zhang - xqzhang@genetics.ac.cn Zan- Min Hu - zmhu@genetics.ac.cn Corresponding author Published 18 March 2008 Received I July 2007 BMC Plant Biology 2008 8 28 doi 10.1186 1471-2229-8-28 Accepted 18 March 2008 This article is available from http www.biomedcentral.cOm 1471-2229 8 28 2008 Zhou et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background To obtain important expressed sequence tags ESTs located on specific chromosomes is currently difficult. Construction of single-chromosome EST library could be an efficient strategy to isolate important ESTs located on specific chromosomes. In this research we developed a method to rapidly isolate ESTs from chromosome 1R of rye by combining the techniques of chromosome microdissection with