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Báo cáo hóa học: " Patients with chronic fatigue syndrome performed worse than controls in a controlled repeated exercise study despite a normal oxidative phosphorylation capacity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Patients with chronic fatigue syndrome performed worse than controls in a controlled repeated exercise study despite a normal oxidative phosphorylation capacity | Vermeulen et al. Journal of Translational Medicine 2010 8 93 http www.translational-medicine.eom content 8 1 93 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Patients with chronic fatigue syndrome performed worse than controls in a controlled repeated exercise study despite a normal oxidative phosphorylation capacity 1 1 1 2 3 Ruud CW Vermeulen Ruud M Kurk Frans C Visser Wim Sluiter Hans R Scholte Abstract Background The aim of this study was to investigate the possibility that a decreased mitochondrial ATP synthesis causes muscular and mental fatigue and plays a role in the pathophysiology of the chronic fatigue syndrome CFS ME . Methods Female patients n 15 and controls n 15 performed a cardiopulmonary exercise test CPET by cycling at a continuously increased work rate till maximal exertion. The CPET was repeated 24 h later. Before the tests blood was taken for the isolation of peripheral blood mononuclear cells PBMC which were processed in a special way to preserve their oxidative phosphorylation which was tested later in the presence of ADP and phosphate in permeabilized cells with glutamate malate and malonate plus or minus the complex I inhibitor rotenone and succinate with rotenone plus or minus the complex II inhibitor malonate in order to measure the ATP production via Complex I and II respectively. Plasma CK was determined as a surrogate measure of a decreased oxidative phosphorylation in muscle since the previous finding that in a group of patients with external ophthalmoplegia the oxygen consumption by isolated muscle mitochondria correlated negatively with plasma creatine kinase 24 h after exercise. Results At both exercise tests the patients reached the anaerobic threshold and the maximal exercise at a much lower oxygen consumption than the controls and this worsened in the second test. This implies an increase of lactate the product of anaerobic glycolysis and a decrease of the mitochondrial ATP production in the patients. In the past this .