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báo cáo hóa học:" Direct effects of caffeine on osteoblastic cells metabolism: the possible causal effect of caffeine on the formation of osteoporosis"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Direct effects of caffeine on osteoblastic cells metabolism: the possible causal effect of caffeine on the formation of osteoporosis | Journal of Orthopaedic Surgery and Research BioMed Central Open Access Direct effects of caffeine on osteoblastic cells metabolism the possible causal effect of caffeine on the formation of osteoporosis Yang-Hwei Tsuang1 Jui-Sheng Sun 1 2 Li-Ting Chen3 Samuel Chung-Kai Sun4 and San-Chi Chen5 Address Department of Orthopedic Surgery Taipei City Hospital Taipei Taiwan ROC institute of Rehabilitation Science and Technology National Yang-Ming University Taipei Taiwan ROC 3HealthBanks Biotechnology Cooperation Limited Taipei Taiwan ROC 4Department of Biochemistry Queen s University Kingston Ontario Canada and 5Department of Orthopedic Surgery Cathay General Hospital Taipei Taiwan ROC Email Yang-Hwei Tsuang - tsuang66@ms71.hinet.net Jui-Sheng Sun - jssun@ym.edu.tw Li-Ting Chen - chenlt@ha.mc.ntu.edu.tw Samuel Chung-Kai Sun - 3scs@qlink.queensu.ca San-Chi Chen - albertscchen@yahoo.com.tw Corresponding author Published 07 October 2006 Received 16 February 2006 Journal of Orthopaedic Surgery and Research 2006 1 7 doi l0.ll86 I749-799X-I-7 Accepted 07 October 2006 This article is available from http www.josr-online.com content 1 1 7 2006 Tsuang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Caffeine consumption has been reported to decrease bone mineral density BMD increase the risk of hip fracture and negatively influence calcium retention. In this study we investigated the influence of caffeine on the osteoblasts behaviour. Method Osteoblasts derived from newborn Wistar-rat calvaria was used in this study. The effects of various concentrations of caffeine on bone cell activities were evaluated by using MTT assay. Alkaline .