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báo cáo hóa học:" Vascular endothelial growth factor regulates osteoblast survival – evidence for an autocrine feedback mechanism"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Vascular endothelial growth factor regulates osteoblast survival – evidence for an autocrine feedback mechanism | Journal of Orthopaedic Surgery and Research BioMed Central Research article Vascular endothelial growth factor regulates osteoblast survival -evidence for an autocrine feedback mechanism John Street 1 2 and Brian Lenehan1 2 Address Department of Orthopedic Surgery National University of Ireland Cork Ireland and 2Combined Neurosurgical and Orthopedic Spine Program University of British Columbia Vancouver BC Canada Email John Street - john.street@vch.ca Brian Lenehan - brian.lenehan@vch.ca Corresponding author Open Access Published 16 June 2009 Journal of Orthopaedic Surgery and Research 2009 4 19 doi l0.ll86 l749-799X-4-l 9 Received 26 February 2009 Accepted 16 June 2009 This article is available from http www.josr-online.com content 4 l l9 2009 Street and Lenehan licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Apoptosis of osteoblasts and osteoclasts regulates bone homeostasis. Skeletal injury in humans results in angiogenic responses primarily mediated by vascular endothelial growth factor VEGF a protein essential for bone repair in animal models. Osteoblasts release VEGF in response to a number of stimuli and express receptors for VEGF in a differentiation dependent manner. This study investigates the putative role of VEGF in regulating the lifespan of primary human osteoblasts PHOB in vitro. Methods PHOB were examined for VEGF receptors. Cultures were supplemented with VEGF 0-50 ng mL a neutralising antibody to VEGF mAB VEGF 0.3 ug mL and Placental Growth Factor PlGF an Flt-l receptor-specific VEGF ligand 0-l00 ng mL to examine their effects on mineralised nodule assay alkaline phosphatase assay and apoptosis. The role