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Báo cáo hóa học: " Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line"

Tham khảo tài liệu 'báo cáo hóa học: " kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line Rosa E Sarmiento Rocio G Tirado Laura E Valverde and Beatriz Gomez-Garcia Address Departamento de Microbiología y Parasitología Facultad de Medicina Universidad Nacional Autónoma de México Ciudad Universitaria D.F. México Email Rosa E Sarmiento - rosass@servidor.unam.mx Rocio G Tirado - tiradom@yahoo.com Laura E Valverde - chanteval@yahoo.com Beatriz Gómez-Garcia - begomez@servidor.unam.mx Corresponding author Published 3 July 2007 Received 2 March 2007 Virology Journal 2007 4 68 doi 10.1186 1743-422X-4-68 Accepted 3 July 2007 This article is available from http www.virologyj.cOm content 4 1 68 2007 Sarmiento et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The binding of viral-specific antibodies to cell-surface antigens usually results in down modulation of the antigen through redistribution of antigens into patches that subsequently may be internalized by endocytosis or may form caps that can be expelled to the extracellular space. Here by use of confocal-laser-scanning microscopy we investigated the kinetics of the modulation of respiratory syncytial virus RSV antigen by RSV-specific IgG. RSV-infected human epithelial cells HEp-2 were incubated with anti-RSV polyclonal IgG and at various incubation times the RSV-cell-surface-antigen-antibody complexes RSV Ag-Abs and intracellular viral proteins were detected by indirect immunoflourescence. Results Interaction of anti-RSV polyclonal IgG with RSV HEp-2 infected cells induced relocalization and aggregation of viral glycoproteins in the plasma membrane formed patches that subsequently .