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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses"

Tham khảo tài liệu 'báo cáo hóa học: " determination of suitable housekeeping genes for normalisation of quantitative real time pcr analysis of cells infected with human immunodeficiency virus and herpes viruses"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Methodology Open Access Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses Sarah Watson1 Sarah Mercier1 Chris Bye2 John Wilkinson3 Anthony L Cunningham1 and Andrew N Harman 1 Address 1Centre for Virus Research Westmead Millennium Institute Sydney Australia 2The Howard Florey Institute University of Melbourne Melbourne Australia and 3Biotron Limited Centre for Immunology St. Vincent s Hospital Sydney Australia Email Sarah Watson - sarahlouise53@hotmail.com Sarah Mercier - sarah_mercier@wmi.usyd.edu.au Chris Bye - cbye@hfi.unimelb.edu.au John Wilkinson - john_wilkinson@cfi.unsw.edu.au Anthony L Cunningham - tony_cunningham@wmi.usyd.edu.au Andrew N Harman - andrew_harman@wmi.usyd.edu.au Corresponding author Published 3 December 2007 Received 10 October 2007 Accepted 3 December 2007 Virologyjournal 2007 4 130 doi l0.ll86 l743-422X-4-130 This article is available from http www.virologyj.cOm content 4 1 130 2007 Watson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections human immunodeficiency virus type 1 herpes simplex virus type 1