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Báo cáo hóa học: " Universal primers that amplify RNA from all three flavivirus subgroups"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Universal primers that amplify RNA from all three flavivirus subgroups | Virology Journal BioMed Central Research Open Access Universal primers that amplify RNA from all three flavivirus subgroups Sheryl L Maher-Sturgess1 Naomi L Forrester2 Paul J Wayper3 Ernest A Gould2 Roy A Hall4 Ross T Barnard4 and Mark J Gibbs 3 Address 1 Australian Biosecurity CRC University of Queensland St Lucia QLD 4067 Australia 2Centre for Ecology and Hydrology Mansfield Rd Oxford Oxfordshire OX13SR UK 3School of Botany and Zoology Australian National University Canberra ACT 0200 Australia and 4School of Molecular and Microbial Sciences University of Queensland St Lucia QLD 4067 Australia Email Sheryl L Maher-Sturgess - Sheryl.Maher@gmail.com Naomi L Forrester - nafores@utmb.edu Paul J Wayper - Paul.Wayper@anu.edu.au Ernest A Gould - eag@ceh.ac.uk Roy A Hall - Roy.Hall@uq.edu.au Ross T Barnard - RossBarnard@uq.edu.au Mark J Gibbs - Mark.Gibbs@anu.edu.au Corresponding author Published 24 January 2008 Virology Journal 2008 5 16 doi 10.1186 1743-422X-5-16 Received 28 November 2007 Accepted 24 January 2008 This article is available from http www.virologyj.cOm content 5 1 16 2008 Maher-Sturgess et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Species within the Flavivirus genus pose public health problems around the world. Increasing cases of Dengue and Japanese encephalitis virus in Asia frequent outbreaks of Yellow fever virus in Africa and South America and the ongoing spread of West Nile virus throughout the Americas show the geographical burden of flavivirus diseases. Flavivirus infections are often indistinct from and confused with other febrile illnesses. Here we review the specificity of published primers and describe a new universal primer pair that can detect a wide range of flaviviruses