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Báo cáo hóa học: " Application of functional genomics to the chimeric mouse model of HCV infection: optimization of microarray protocols and genomics analysis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Application of functional genomics to the chimeric mouse model of HCV infection: optimization of microarray protocols and genomics analysis | Virology Journal BioMed Central Methodology Open Access Application of functional genomics to the chimeric mouse model of HCV infection optimization of microarray protocols and genomics analysis Kathie-Anne Walters 1 Michael A Joyce2 Jill C Thompson1 Sean Proll1 James Wallace1 Maria W Smith1 Jeff Furlong1 D Lorne Tyrrell2 and Michael G Katze1 Address Department of Microbiology University of Washington Seattle WA USA and 2Department of Medical Microbiology and Immunology University of Alberta Edmonton Alberta Canada Email Kathie-Anne Walters - kathiw@u.washington.edu Michael A Joyce - maj2@ualberta.ca Jill C Thompson - jcthom@u.washington.edu Sean Proll - proll@u.washington.edu James Wallace - jwallace@u.washington.edu Maria W Smith - smitmv@u.washington.edu Jeff Furlong - jfurlong@u.washington.edu D Lorne Tyrrell - ltyrrell@med.ualberta.ca Michael G Katze - honey@u.washington.edu Corresponding author Published 25 May 2006 Received 10 April 2006 Accepted 25 May 2006 Virology Journal 2006 3 37 doi 10.1 186 1743-422X-3-37 This article is available from http www.virologyj.cOm content 3 1 37 2006 Walters et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Many model systems of human viral disease involve human-mouse chimeric tissue. One such system is the recently developed SCID-beige Alb-uPA mouse model of hepatitis C virus HCV infection which involves a human-mouse chimeric liver. The use of functional genomics to study HCV infection in these chimeric tissues is complicated by the potential cross-hybridization of mouse mRNA on human oligonucleotide microarrays. To identify genes affected by mouse liver mRNA hybridization mRNA from identical human liver samples labeled with either Cy3 or Cy5 was compared .