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báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2 | Berencsi et al. Journal of Translational Medicine 2011 9 33 http www.translational-medicine.eom content 9 1 33 TRANSLATIONAL MEDICINE RESEARCH Open Access In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2 1 1 1 1 2 3.4 Klara Berencsi Pyapalli Rani Tianqian Zhang Laura Gross Michael Mastrangelo Neal J Meropol Dorothee Herlyn1 and Rajasekharan Somasundaram1 Abstract Background Infiltration of colorectal carcinomas CRC with T-cells has been associated with good prognosis. There are some indications that chemokines could be involved in T-cell infiltration of tumors. Selective modulation of chemokine activity at the tumor site could attract immune cells resulting in tumor growth inhibition. In mouse tumor model systems gene therapy with chemokines or administration of antibody Ab -chemokine fusion proteins have provided potent immune mediated tumor rejection which was mediated by infiltrating T cells at the tumor site. To develop such immunotherapeutic strategies for cancer patients one must identify chemokines and their receptors involved in T-cell migration toward tumor cells. Methods To identify chemokine and chemokine receptors involved in T-cell migration toward CRC cells we have used our previously published three-dimensional organotypic CRC culture system. Organotypic culture was initiated with a layer of fetal fibroblast cells mixed with collagen matrix in a 24 well tissue culture plate. A layer of CRC cells was placed on top of the fibroblast-collagen layer which was followed by a separating layer of fibroblasts in collagen matrix. Anti-CRC specific cytotoxic T lymphocytes CTLs mixed with fibroblasts in collagen matrix were placed on top of the separating layer. Excess chemokine ligand CCL or Abs to chemokine or chemokine receptor CCR were used in migration inhibition assays to identify the chemokine and the receptor involved in CTL migration. Results Inclusion of excess CCL2 in T-cell layer or Ab