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o cáo hóa học:" Peritoneal carcinomatosis from ovarian cancer: chemosensitivity test and tissue markers as predictors of response to chemotherapy"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Peritoneal carcinomatosis from ovarian cancer: chemosensitivity test and tissue markers as predictors of response to chemotherapy | Arienti et al. Journal of Translational Medicine 2011 9 94 http www.translational-medicine.eom content 9 1 94 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Peritoneal carcinomatosis from ovarian cancer chemosensitivity test and tissue markers as predictors of response to chemotherapy 1 -I--1 2 2 3 3 Chiara Arienti Anna Tesei Giorgio Maria Verdecchia Massimo Framarini Salvatore Virzi Antonio Grassi 11 1 1 1 Emanuela Scarpi Livia Turci Rosella Silvestrini Dino Amadori and Wainer Zoli Abstract Background Platinum-based regimens are the treatments of choice in ovarian cancer which remains the leading cause of death from gynecological malignancies in the Western world. The aim of the present study was to compare the advantages and limits of a conventional chemosensitivity test with those of new biomolecular markers in predicting response to platinum regimens in a series of patients with peritoneal carcinomatosis from ovarian cancer. Methods Fresh surgical biopsy specimens were obtained from 30 patients with primary or recurrent peritoneal carcinomatosis from ovarian cancer. ERCC1 GSTP1 MGMT XPD and BRCA1 gene expression levels were determined by Real-Time RT-PCR. An in vitro chemosensitivity test was used to define a sensitivity or resistance profile to the drugs used to treat each patient. Results MGMT and XPD expression was directly and significantly related to resistance to platinum-containing treatment p 0.036 and p 0.043 respectively . Significant predictivity in terms of sensitivity and resistance was observed for MGMT expression 75.0 and 72.5 respectively p 0.03 while high predictivity of resistance 90.9 but very low predictivity of sensitivity 37.5 p 0.06 were observed for XPD. The best overall and significant predictivity was observed for chemosensitivity test results 85.7 sensitivity and 91.3 resistance p 0.0003 . Conclusions The in vitro assay showed a consistency with results observed in vivo in 27 out of the 30 patients analyzed. Sensitivity and .