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báo cáo hóa học: " Influence of dietary state and insulin on myocardial, skeletal muscle and brain [18F]fluorodeoxyglucose kinetics in mice"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Influence of dietary state and insulin on myocardial, skeletal muscle and brain [18F]fluorodeoxyglucose kinetics in mice | Kreissl et al. EJNMMI Research 2011 1 8 http www.ejnmmires.eom content 1 1 8 9 EJNMMI Research a SpringerOpen Journal ORIGINAL RESEARCH Open Access Influence of dietary state and insulin on myocardial skeletal muscle and brain 18F -fluorodeoxyglucose kinetics in mice 1.2 3 114 3 Michael C Kreissl 1 David B Stout Koon-Pong Wong Hsiao-Ming Wu Evren Caglayan Waldemar Ladno 1 1.5 2 1 1 Xiaoli Zhang John O Prior Christoph Reiners Sung-Cheng Huang and Heinrich R Schelbert Abstract Background We evaluated the effect of insulin stimulation and dietary changes on myocardial skeletal muscle and brain 18F -fluorodeoxyglucose FDG kinetics and uptake in vivo in intact mice. Methods Mice were anesthetized with isoflurane and imaged under different conditions non-fasted n 7 controls non-fasted with insulin 2 lU kg body weight injected subcutaneously immediately prior to FDG n 6 fasted n 5 and fasted with insulin injection n 5 . A 60-min small-animal PET with serial blood sampling and kinetic modeling was performed. Results We found comparable FDG standardized uptake values SUVs in myocardium in the non-fasted controls and non-fasted-insulin injected group SUV 45-60 min 9.58 1.62 vs. 9.98 2.44 p 0.74 a lower myocardial SUV was noted in the fasted group 3.48 1.73 p 0.001 . In contrast the FDG uptake rate constant Kj for myocardium increased significantly by 47 in non-fasted mice by insulin 13.4 3.9 ml min 100 g vs. 19.8 3.3 ml min 100 g p 0.030 in fasted mice a lower myocardial Kị as compared to controls was observed 3.3 1.9 ml min 100 g p 0.001 . Skeletal muscle SUVs and Kị values were increased by insulin independent of dietary state whereas in the brain those parameters were not influenced by fasting or administration of insulin. Fasting led to a reduction in glucose metabolic rate in the myocardium 19.41 5.39 vs. 3.26 1.97 mg min 100 g p 0.001 the skeletal muscle 1.06 0.34 vs. 0.34 0.08 mg min 100 g p 0.001 but not the brain 3.21 0.53 vs. 2.85 0.25 mg min 100 g p 0.19 . .