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Replacing Fertiliser N with Rhizobial Inoculants for Legumes in Vietnam for Greater Farm Profitability and Environmental Benefits

The overall objective of the project is to reduce the use of N fertilizer use on legumes soybean and groundnut in Vietnam through by increasing legume inoculants. Replace N fertilizer with legume inoculants will provide both economic benefits and environmental benefits for farmers and reduce financial stress for poor farmers by reducing input costs. The approach is to raise awareness and demand for inoculants through an expanded program including live demonstrations of the benefits of inoculants and training in the functions and their use. Also, a technical program targeted production capacity of high quality inoculants in Vietnam has been started | Project Number 013 06VIE Replacing Fertiliser N with Rhizobial Inoculants for Legumes in Vietnam for Greater Farm Profitability and Environmental Benefits QUALITY CONTROL OF LEGUME INOCULANTS WORKSHOP 26th February - 9th March 2007 Presented by E. Hartley R. Deaker G. Gemell WORKBOOK Compiled by E. Hartley G. Gemell J. Hartley NSW DEPARTMENT OF PRIMARY INDUSTRIES Australian Legume Inoculants Research Unit An NCSI QMS ISO9001 2000 Certified Laboratory 9187-21 Version date 1 2 2007 WORKSHOP SCHEDULE WEEK 1 WEEK 1 WEEK 1 WEEK 1 WEEK 1 MONDAY 26th TUESDAY 27th WEDNESDAY 28th THURSDAY 1st FRIDAY 2nd SAT RHIZOBIAL STRAINS QC STRAINS AND STRAIN AUTHENTICITY STRAIN MAINTENANCE CONTROLLED RELEASE OF STRAINS CHECK SEROLOGIES FROM YESTERDAY. DISCUSS PLANT GROWTH ASSEMBLIES AND ALTERNATIVES LECTURE ON RHIZOBIUM SYMBIOSIS INCEPTION WORKSHOP -CONTINUED EXAMINE THE TA1 CULTURES ON STREAKED PLATES AND AGAR SLOPES FOR RHIZOBIA MORPHOLOGY AND ANY CONTAMINANTS GRAM STAIN INOCULANTS QC QUALITY AND TYPES OF CARRIER NUMBERS RHIZOBIA GRAM CARRIER PURITY TEST FOR CONTAMINANTS MOISTURE CONTENT OF CARRIER AND EFFECTS ON RHIZOBIA SETTING ACCEPTABLE STANDARDS PROCEDURES TO ACHIEVE AND CONTROL QUALITY OF INOCULANTS MEDIA PREP N ASSEMBLIES PETRI PLATES MC BOTTLES BROTH FLASKS PLANT GROWTH TUBES QC PROCEDURES LIST WOKSHOP BEGINS HANDS ON -FOR DISCUSSION -STERILISATION EQUIPMENT AT EACH INSTITUTE SUPPLIES EQUIPMENT STORAGE OF MEDIA ETC. WHAT ALIRU DOES CONSIDER HOW IT MIGHT BE DIFFERENT AT OTHER LABS. MAKING BROTH DILUENTS. LUNCH SURFACE STERILIZATION OF SEEDS INCEPTION WORKSHOP INCEPTION WORKSHOP BROTH CULTURES PLATED ONTO AGAR PLATES INOCULATION OF BROTHS FROM SUPPLIED CULTURES GROWN ON AGAR SLOPES McCARTNEYS INCEPTION WORKSHOP INCEPTION WORKSHOP INOCULATE PRE-SOWN TEST HOST PLANTS WITH RESPECTIVE BROTH CULTURES FOR NODULATION CHECK LUNCH LUNCH INCEPTION WORKSHOP INCEPTION WORKSHOP LUNCH LUNCH GROUP EXERCISES DIVIDE INTO 3 GROUPS - PRACTICAL WORK RHIZOBIAL STRAIN QUALITY -SUBCULTURING OF 5 .