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Báo cáo hóa học: " Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface | Nanoscale Res Lett 2009 4 1403-1408 DOI 10.1007 S11671-009-9412-9 NANO EXPRESS Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface Zhengjian Lv Jianhua Wang Linhong Deng Guoping Chen Received 24 June 2009 Accepted 7 August 2009 Published online 16 September 2009 to the authors 2009 Abstract The 16-mercaptohexadecanoic acid MHA film and rat anti-human IgG protein monolayer were fabricated on gold substrates using self-assembled monolayer SAM method. The surface properties of the bare gold substrate the MHA film and the protein monolayer were characterized by contact angle measurements atomic force microscopy AFM grazing incidence X-ray diffraction GIXRD method and X-ray photoelectron spectroscopy respectively. The contact angles of the MHA film and the protein monolayer were 18 and 12 respectively all being hydrophilic. AFM images show dissimilar topographic nanostructures between different surfaces and the thickness of the MHA film and the protein monolayer was estimated to be 1.51 and 5.53 nm respectively. The GI-XRD 20 degrees of the MHA film and the protein monolayer ranged from 0 to 15 significantly smaller than that of the bare gold surface but the MHA film and the protein monolayer displayed very different profiles and distributions of their diffraction peaks. Moreover the spectra of binding energy measured from these different surfaces could be well fitted with either Au4f S2p or N1s respectively. Taken together these results indicate that MHA film and protein monolayer were successfully formed with homogeneous surfaces and thus demonstrate that the SAM method is a reliable technique for fabricating protein monolayer. Z. Lv J. Wang El L. Deng G. Chen Key Laboratory of Biorheological Science and Technology Ministry of Education and Institute of Biochemistry and Biophysics College of Bioengineering Chongqing University 400044 Chongqing China e-mail wjh@cqu.edu.cn G. Chen e-mail .