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Culture of animal cells

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Tissue culture was first devised at the beginning of the twentieth century [Harrison, 1907; Carrel, 1912] (Table 1.1) as a method for studying the behavior of animal cells free of systemic variations that might arise in vivo both during normal homeostasis and under the stress of an experiment. As the name implies, the technique was elaborated first with undisaggregated fragments of tissue, and growth was restricted to the migration of cells from the tissue fragment, with occasional mitoses in the outgrowth. As culture of cells from such primary explants of tissue dominated the field for more than 50 years [Fischer, 1925; Parker, 1961], it is not surprising that the name ‘‘tissue.

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