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Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties

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Periplasmic binding proteins are abundant in bacteria by virtue of their essential roles as high-affinity receptors in ABC transport systems and chemotaxis. One of the best studied of these receptors is the so-called glucose⁄galactose-binding protein. Here, we report the X-ray structure of the Salmonella typhimuriumprotein bound to the physiologically relevant ligand, (2R)-glyceryl-b-d-galactopyranoside, solved by molecular replace-ment, and refined to 1.87 A˚ resolution with RandR-free values of 17% and 22%

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