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Báo cáo khoa học: Usefulness of microchip electrophoresis for reliable analyses of nonstandard DNA samples and subsequent on-chip enzymatic digestion

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The Hitachi SV1100 utilizes capillary electrophoresis on a microchip that is capable of rapidly sizing DNAfrag-ments. Reproducibility of electrophoresis in different channels was shown by comparing the migration times of the internal controls, DNAfragments of 100 and 800 bp. The range of DNAsizing for this microchip is between 100 and 800 bp, and accuracy in sizing of a 322 bp DNA fragment of a pUC118 PvuII digest was observed, inde-pendent of DNAconcentration. Although relatively good quantification of this fragment was observed with a DNA concentration of 1.83 ngÆlL )1 , error increased in a dose-dependent manner. .

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