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Lecture Principles of biochemistry - Chapter 7 (part 2): Cofactors

This chapter help you: Be able to recognize (not draw) all coenzymes (NAD(P)+/NAD(P) H, FAD+/FADH, FMN, thiamin, pyridoxal phosphate, biotin, tetrahydrofolate, cobalamin, Coenzyme A, Lipoamide, Vitamin K, Ubiquinone, plastoquinone). Also be able to identify functional chemical groups in overall structure. Know in what type of RXN each coenzyme participates. | Chapter 7 (part 2) Cofactors Biotin Water soluble Vitamin Produced by gut microflora which supplies ½ RDA Deficiencies are rare Consuming 6 raw eggs a day can cause deficiencies due to the presence avidin (biotin binding protein). Biotin cofactor Involved in ATP dependent carboxylation rxns Covalently bound to enzyme through amide linkage w/lysine Impt. Biotin enzymes = acetyl-CoA carboxylase, pyruvate carboxylase Folate Water soluble vitamin Folate impt. during pregnancy to prevent neural tube defects in fetus (I.e. spina bifida) Vitamin B12 deficiencies cause folate deficiencies Has a poly-glutamate tail formed by gamma-carboxy and alpha amino groups (unusual peptide bond). pterin PABA glutamate Tetrahydrofolate (THF) Folate is converted to THF by the addition of 4 hydrogens to the pterin ring. Impt. in transfer of one-carbon units Pterin ring impt. functional group Can transfer one carbon units at the oxidation level of methanol, formaldehyde, or formic acid. Cobalamin (B12) Water soluble Vitamin Corrin ring with Cobalt cation Involved in intramolecular rearrangements, methyl group transfer, reduction of ribonucleotides to deoxyribonucleotides. Forms radical species Pantothenic acid/Coenzyme A (CoA) Pantothenic acid is water soluble vitamin Co-enzyme A involved in acyl group transfer Sulfhydroyl group impt. Hydrophobic acyl groups (fatty acids) are made more water soluble w/CoA attached Lipoic acid/Lipoamide Not a vitamin Important reactive groups are the sulfur atoms Disulfide can be reduced to form 2 sulfhydryl groups Involved in acyl group transfer reactions Co-factor covalently attached to enzyme through amide linkage with lysine residue Lipoamide Fat soluble Vitamins Vitamin A (retinol) derived from b-carotene impt for vision, regulation of gene expression during cell differentiation, teratogenic Vitamin D – impt in Ca absorption, regulates intestinal absorption and deposition in bones Vitamin E – antioxidant reduced form oxidized form Vitamin K is a cofactor for the enzyme that carboxylates certain glutamate residues on prothrombin to g-carboxyglutamate residues. Ca+ binds g-carboxyglutamate residues causes protein to adhere to platelet surface Only fat soluble cofactor that functions as a cofactor Drugs inhibit reduction of oxidized form of vitamin K Ubiquinone/Plastoquinone Lipid soluble electron carriers. Impt in electron transport chains Can accept or donate electrons one or two at a time Protein coenzymes Usually small proteins Active groups are either prosthetic groups or part of protein backbone Participate in group transfer and oxidation/reduction rxns acyl carrier protein biotin carboxyl carrier protein cytochromes Protein coenzyme Heme containing proteins Fe3+ can undergo reversible one electron reduction Impt in redox rxns Classified based on the basis of their visible absorbance spectra