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Antioxidant and hypoglycemic activities of extract and fractions of Rambutan seeds

The ethanol extract of rambutan seeds were prepared by maseration method and the fractions (nhexane, ethyl acetate, buthanol and water) by separation of extract based on the polarity. The antioxidant activity was determined by using superoxide dismutase value (SOD), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity,. Invite you to consult the documentation. | S. Soeng, E. Evacuasiany, W. Widowati, N. Fauziah. Biomedical Engineering Vol.1 No.1 (2015) 13 BE BIOMEDICAL ENGINEERING journal homepage: be.ub.ac.id Antioxidant and hypoglycemic activities of extract and fractions of Rambutan seeds (Nephelium lappaceum L.) Slyvia Soeng1†, Endang Evacuasiany1, Wahyu Widowati1†, Nurul Fauziah2 1 Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia 2 ARTICLE INFO Article history: Received: January 2015 Accepted: June 2015 Available online: August 2015 Keywords: α-glucosidase Antidiabetic Antioxidant DPPH Nephelium lappaceum L. SOD † Corresponding author: Jl. Prof. Drg. Suria Sumantri no 65 Bandung 40164, Indonesia. wahyu_w60@yahoo.com; s.soeng@yahoo.com ABSTRACT Objective : This research was done to measure the antioxidants and hypoglycemic activities of NLS extract and fractions. Methods: The ethanol extract of rambutan seeds were prepared by maseration method and the fractions (nhexane, ethyl acetate, buthanol and water) by separation of extract based on the polarity. The antioxidant activity was determined by using superoxide dismutase value (SOD), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. The hypoglycemic activity was determined by using inhibition of α-glucosidase test. The DPPH scavenging and hypoglycemic activities were analized by median of Inhibitory Concentration (IC-50). Result : The highest SOD activity showed that ethyl acetate and aqueous fraction of NLS were 3.3771 µg/ml and 3.0374 µg/ml. Meanwhile DPPH assay showed that both NLS extract and fractions had low DPPH scavenging activity. Hypoglycemic activity showed that extract of NLS had highest activity as α-glucosidase inhibitor. Conclusion : NLS extract and fractions have high SOD antioxidant value but low DPPH scavenging activity and can be used as potential hypoglycemic agent. 1. Introduction Diabetes .