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Fermentative biohydrogen production by anaerobic, thermophilic bacterium thermoanaerobacterium aciditolerans Trau Dat isoalted

Thermoanaerobacterium aciditolerans bacterium Trau DAt isolated in Vietnam had the hydrogen production capability under the anaerobic condition at 55oC. Using glucose, the dark fermentation of strain Trau DAt for hydrogen production was performed in three different scales: the flask scales under the suitable and optimal conditions, and the automatic fermentor system Bio-Flo 110. | TAP CHI SINH HOC 2014, 36(4): 515-521 Fermentative biohydrogen production by anaerobic DOI: 10.15625/0866-7160/v36n4.6181 FERMENTATIVE BIOHYDROGEN PRODUCTION BY ANAEROBIC, THERMOPHILIC BACTERIUM Thermoanaerobacterium aciditolerans Trau DAt ISOALTED FROM VIETNAM Nguyen Thi Yen1, Lai Thuy Hien 1, Nguyen Thi Thu Huyen1,2* 1 Institute of Biotechnology, Vietnam Academy of Science and Technology 2 Nguyen Tat Thanh University, *huyen308@gmail.com ABSTRACT: Thermoanaerobacterium aciditolerans bacterium Trau DAt isolated in Vietnam had the hydrogen production capability under the anaerobic condition at 55oC. Using glucose, the dark fermentation of strain Trau DAt for hydrogen production was performed in three different scales: the flask scales under the suitable (1) and optimal (2) conditions, and the automatic fermentor system Bio-Flo 110 (3). Under the suitable condition, the strain Trau DAt produced 330 ml volume total gas (L-1) and hydrogen occupied 42.95% of gas total. Under the optimal condition, the maximum volume total gas of 701 ml (L-1) was obtained and hydrogen occupied of 77.2% total volume gas. Based on results of RSM analysis and pH controlled examination, the dark fermentation of strain Trau DAt was performed under automatic fermentor system scale (Bio-Flo 110). 2.64 L total gas (L-1) was obtained by consuming 92.58% glucose and hydrogen volume occupied 94.85% of gas total. The maximum hydrogen yield of strain Trau DAt was 1.63 mol H2 (mol glucose)-1. Obtained results showed the remarkable potentiality of Trau DAt strain in application to the larger fermentation scale for biohydrogen production in Vietnam. Keywords: Thermoanaerobacterium aciditolerans, anaerobic, biohydrogen, dark fermentation, thermophilic bacteria, Vietnam. INTRODUCTION Hydrogen is widely recognized as a clean and efficient energy resource for future. It is the only common fuel that is not chemically bound to carbon. When hydrogen burns in air, it gives off nothing worse than water .