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Investigation of the ability to stimulate immune response by using complex oncolytic virus Measle/Mump in nude mouse model bearing human colon cancer

Objectives: The present study uses immune-deficient mice model bearing human colon cancer to investigate the change of the rate of immune cells which play a key role in cancer treatment by treating complex oncolytic virus (OLV) Measle/Mump. | Journal of military pharmaco-medicine No7-2016 INVESTIGATION OF THE ABILITY TO STIMULATE IMMUNE RESPONSE BY USING COMPLEX ONCOLYTIC VIRUS MEASLE/MUMP IN NUDE MOUSE MODEL BEARING HUMAN COLON CANCER Le Duy Cuong*; Nguyen Phuong Thanh**; Nguyen Dac Tu** Nguyen Linh Toan*; Ho Anh Son* SUMMARY Objectives: The present study uses immune-deficient mice model bearing human colon cancer to investigate the change of the rate of immune cells which play a key role in cancer treatment by treating complex oncolytic virus (OLV) Measle/Mump. Subject and methods: Nude mice was divided into 4 groups: control group, measle virus-treated group, mump virus-treated group and complex Measle/Mump virus-treated group with dose of 106 CFU/individual for single injection. Population of macrophage, dendritic cell, NK cell in nude mice’s spleen were analyzed by flow cytometry method. Results: The results showed differences between groups: the rate of immune cells in treated groups were clearly increased compared to control group while complex OLV-treated group had a higher rate of immune cells than single OLV-treated groups. Conclusion: Using complex OLV bring a significant effects on stimulating immune response more than using single OLV. * Key words: Colon cancer; Oncolytic virus Measle/Mump; Immune response; Nude mice. INTRODUCTION Human colon cancer has been known for a long time as a common type of cancer. In Vietnam, the prevalence of cancer patients tends to be increased annually. Recently, scientists have created some new kinds of medicines in colon cancer treatment which are not only more effective but also decrease side effects. However, they’re still looking for a new method with optimizing ability of selectively killing cancer cells and minimizing the damage to normal cells. They give a theory that oncolytic virotherapy can solve this problem. Oncolytic virotherapy have been used since the late 20 th century. Dramatic achievements of molecular biology had shed light on the .