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The drought tolerant characteristics of some upland local maize cultivars (Zea Mays L.) in the north of Viet Nam

Maize (Zea mays L.) is one of the widely grown grains in Viet Nam. The local upland maize cultivars play an important role for the life of people living in mountainous regions in the North of Viet Nam. The quality of seed and drought tolerance ability of local upland maize cultivars are good. In this work, we have studied drought tolerance character and dehydrin gene of some local upland maize cultivars. | Chu Hoàng Mậu và cs Tạp chí KHOA HỌC & CÔNG NGHỆ 77(01): 77 - 82 THE DROUGHT TOLERANT CHARACTERISTICS OF SOME UPLAND LOCAL MAIZE CULTIVARS (ZEA MAYS L.) IN THE NORTH OF VIET NAM Chu Hoang Mau1*, Nguyen Vu Thanh Thanh2, Pham Thi Thanh Nhan1 1* Department of Genetics and Modern Biology, College of Education - TNU 2 Department of Genetics, College of Science - TNU SUMMARY Maize (Zea mays L.) is one of the widely grown grains in Viet Nam. The local upland maize cultivars play an important role for the life of people living in mountainous regions in the North of Viet Nam. The quality of seed and drought tolerance ability of local upland maize cultivars are good. In this work, we have studied drought tolerance character and dehydrin gene of some local upland maize cultivars. Among them these are six cultivars with high drought tolerance ability (BK, HL, QH, YM, TL, and QU) and two maize cultivars BG and VN2 with low drought tolerance ability. Dehydrin – LEA (lete embryogenesis abundant) – D11 is a plant protein family in a number of higher plants, a species of protein produced by plants during drought or low temperature stress. These are six dehydrin protein types of maize: dhn1, dhn2, dhn3, dhn4, dhn5, and dhn6. DHNs are unified by the presence of one or more copies of a putative amphipathic α helix-forming domain (the K-segment), which is highly conserved in higher and lower plants and has a 15 residue consensus sequence EKKGIMDKIKEKLPG. We had cloned dhn1 gene from DNA of genome of local upland maize cultivars. The 0.6 kb of dhn1 gene was amplified by PCR and carry out successfully with LeaZeM1 primers. Cloning of PCR products and DNA sequencing is carrying out. Keywords: Dehydrin, drought, upland local, maize,Dhn1, cloning, PCR INTRODUCTION Maize is widely cultivated throughout the world. Maize (Zea mays L.) is also one of the widely grown grains in Viet Nam. The local upland maize cultivars play an important role for the life of people living in .