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Isolation and identification of aerobic microbial community treating benzene from a constructed wetland system

The main objective of this study was to isolate and identify the aerobic bacteria in bacterial community of constructed wetland in Vietnam, which were spiked with benzene. For this target, four random samples of rhizosphere soil of phragmiteskarkas from constructed wetlands in Vietnam were collected and mixed together. | Journal of Science and Technology 54 (4B) (2016) 102-108 ISOLATION AND IDENTIFICATION OF AEROBIC MICROBIAL COMMUNITY TREATING BENZENE FROM A CONSTRUCTED WETLAND SYSTEM Tran Hau Vuong1, Florencio Ballesteros Jr2, Hoang Trong Khiem1, Nguyen Trung Thao1, Le Thi Phung1 1 Hochiminh University of Natural Resources and Environment, 236B Le Van Sy, Ward 1, Tan Binh District, Ho Chi Minh City 2 University of the Philippines Diliman, Quezon City 1101, Metro Manila, Philippine Received: 15th August 2016; Accepted for publication: 10th November 2016 ABSTRACT The main objective of this study was to isolate and identify the aerobic bacteria in bacterial community of constructed wetland in Vietnam, which were spiked with benzene. For this target, four random samples of rhizosphere soil of phragmiteskarkas from constructed wetlands in Vietnam were collected and mixed together. The soil sample was labeled VS. Bacteria in the sample was grown in 80 mg/L benzene liquid media with optimal conditions of temperature and shaken to find out whether bacteria in the sample can remove benzene or not. The result showed that bacteria in the sample can survive and treat benzene well. Strains of bacteria were isolated through incubation in Burk’s medium mixed with benzene. The strain named VS1 from VS sample was obtained. The strain adapted well in agar medium containing benzene as sole carbon source. Through molecular methods, VS1 was identified as PandoraeapnomenusaCM25. Keywords: benzene treatment, Pandoraeapnomenusa CM25, Wetland, 16S rDNA. 1. INTRODUCTION Benzene is a component of gasoline. It is often found at sites contaminated with petroleum product releases (5). Benzene has been used in industrial syntheses of plastics, paints, pesticides, resins, dyes and solvents of chemicals (18). It is a toxicant and is classified as one of the most important pollutants regulated by the Environmental Protection Agency (6). It causes various human diseases, such as cancer, hematological .