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Clonal propagation and synthetic seed production from nodal segments of Cape gooseberry (Physalis peruviana L.), a tropical fruit plant

Physalis peruviana L. contains polyphenols and carotenoids with antiinflammatory and antioxidant activities used against diabetes. To establish an efficient regeneration system using nodal segments excised from 4-week-old germinated seedlings, direct plant regeneration. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 797-806 © TÜBİTAK doi:10.3906/tar-1412-86 http://journals.tubitak.gov.tr/agriculture/ Research Article Clonal propagation and synthetic seed production from nodal segments of Cape gooseberry (Physalis peruviana L.), a tropical fruit plant 1, 2 2 2 Bahtiyar Buhara YÜCESAN *, Aliyu MOHAMMED , Merve ARSLAN , Ekrem GÜREL Department of Seed Science and Technology, Faculty of Natural and Agricultural Sciences, Abant İzzet Baysal University, Bolu, Turkey 2 Department of Biology, Faculty of Arts and Sciences, Abant İzzet Baysal University, Bolu, Turkey 1 Received: 22.12.2014 Accepted/Published Online: 16.01.2015 Printed: 30.09.2015 Abstract: Physalis peruviana L. contains polyphenols and carotenoids with antiinflammatory and antioxidant activities used against diabetes. To establish an efficient regeneration system using nodal segments excised from 4-week-old germinated seedlings, direct plant regeneration, without additional rooting stage, was achieved on LS medium containing 0.5 mg/L 6-benzylaminopurine (BAP), kinetin (KIN), thidiazuron (TDZ), or gibberellic acid (GA3), alone or in combination with 0.25 mg/L indole-3-acetic acid (IAA) or indole-3butyric acid (IBA), after 2 weeks of incubation. The highest mean numbers of shoots and well-developed roots were obtained on LS medium containing solely 0.5 mg/L TDZ, producing 5.3 shoots and 3.3 roots per explant after 2 weeks of incubation. Direct shoot and root formation were also recorded on LS medium containing no plant growth regulators. Due to the high regeneration capacity of nodal segments, synthetic seed production was also investigated using the sodium alginate (NaAlg) encapsulation technique. Four different matrix compositions, including NaAlg with or without LS medium containing 3% (w/v) sucrose alone or in combination with 0.5 mg/L abscisic acid (ABA) as a growth retardant were tested for the regrowth performance of synthetic seeds