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The novel methods of development of the maintainer and cytoplasmic male sterile lines with different cytoplasms based on chromosome single-segment substitution lines in rice

The present study was carried out with the objective of developing new parental lines of hybrid rice based on chromosome single-segment substitution lines (SSSLs), on which the restorer and maintainer genes Rf3 (rf3) and Rf4 (rf4) were harbored in rice. | Turkish Journal of Agriculture and Forestry http://journals.tubitak.gov.tr/agriculture/ Research Article Turk J Agric For (2014) 38: 441-446 © TÜBİTAK doi:10.3906/tar-1308-75 The novel methods of development of the maintainer and cytoplasmic male sterile lines with different cytoplasms based on chromosome single-segment substitution lines in rice 1,2 1, Jian CAI , Guiquan ZHANG * School of Life Science, Fu Yang Teachers College, Fu Yang, Anhui, P.R. China 2 The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, P.R. China 1 Received: 25.08.2013 Accepted: 12.12.2013 Published Online: 27.05.2014 Printed: 26.06.2014 Abstract: The present study was carried out with the objective of developing new parental lines of hybrid rice based on chromosome single-segment substitution lines (SSSLs), on which the restorer and maintainer genes Rf3 (rf3) and Rf4 (rf4) were harbored in rice. To develop the new 3-line hybrid, the SSSL W23-19-06-06-11 with the genotype Rf3Rf3/Rf4Rf4 [a strong restorer line for wild abortive (WA), dwarf-wild abortive (DA), and Yegong (Y) cytoplasmic male sterile (CMS) lines] was crossed 4 times as the male parent with 4 CMS lines: 2 typical WA-type CMS lines of Bobai A and Zhenshan 97A, 1 typical Y-type CMS line of Y-Huanong A, and 1 typical DA-type CMS line of Xieqingzao A. In the BC3F2 populations, the male sterile plants (MSPs) with WA-, Y-, and DA-type CMS sources and nuclear background of W23-19-06-06-11 and their maintainer plants (MPs) were developed by backcrossing and marker-assisted selection. All the MSPs showed 100% pollen and spikelet sterility and the panicle length ranged from 20 to 22.6 cm. The extent of incomplete panicle exertion in the entries ranged from –40.7 to –48.5. The pollen grain viability assay with I2KI of the MSPs revealed 100% pollen sterility, consisting of both typical and spherical abortive pollen grains. The MPs showed normal