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Identification of potato Y Potyvirus (PVY) resistance in wild and cultivated tomatoes

Potato Y potyvirus (PVY) is an important plant pathogen worldwide that infects and causes yield losses in the family Solanaceae including potato (Solanum tuberosum), pepper (Capsicum spp.), tomato (S. lycopersicum), and tobacco (Nicotiana tabacum). In this study, 20 different tomato accessions representing 6 different species were mechanically inoculated with PVY. | Turk J Agric For 33 (2009) 11-17 © TÜBİTAK doi:10.3906/tar-0804-17 Identification of Potato Y Potyvirus (PVYO) Resistance in Wild and Cultivated Tomatoes Fevziye ÇELEBİ TOPRAK1,*, Eminur BARUTÇU2, Anne FRARY2, Sami DOĞANLAR2 1 Department of Biology, Pamukkale University, Kinikli Merkez Kampus, 20017 Kinikli, Denizli - TURKEY 2 Department of Molecular Biology & Genetics, İzmir Institute of Technology, 35430 Urla, İzmir - TURKEY Received: 14.04.2008 Abstract: Potato Y potyvirus (PVY) is an important plant pathogen worldwide that infects and causes yield losses in the family Solanaceae including potato (Solanum tuberosum), pepper (Capsicum spp.), tomato (S. lycopersicum), and tobacco (Nicotiana tabacum). In this study, 20 different tomato accessions representing 6 different species were mechanically inoculated with PVYO. The plants were scored visually for symptoms and then tested for presence of the virus 2-4 weeks after inoculation by ELISA. The results O were variable. Most wild species of tomato sustained PVY replication in inoculated leaves. Some of the wild species showed an immune response, while some became systemically infected. Inoculation and analysis of F2 populations suggested that the resistance is controlled by a single recessive gene in different wild species. O Key Words: Immunity, resistance, PVY , tomato Yabani ve Kültür Domateslerinde Patates Y Potyvirüsüne (PVYO) Karşı Dayanıklılığın Belirlenmesi Özet: Patates Y potyvirüs (PVY) bütün dünyada çok önemli olan bir bitki patojenidir. Solanaceae familyasına ait olan patates (Solanum tuberosum), biber (Capsicum spp), domates (S. lycopersicum) ve tütün’de (Nicotiana tabacum) önemli ürün kayıplarına sebep O olmaktadır. Bu çalışmada, altı farklı türden her biri farklı sayıda 20 farklı domates çeşit/hat örnekleri PVY ile mekanik olarak inokule edilmiştir. Bitkilere inokulasyondan 2-4 hafta sonra virüs varlığının belirlenmesi için ELISA testi uygulanmıştır ve bitkilerin semptomlarına bakılmıştır. .